Dendritic Filopodia Motility Analyser

An ImageJ/Fiji plugin for the evaluation of live cell image series of dendritic protrusions


Krisztián Tárnok1, Márton Gulyás1, Norbert Bencsik1, Katalin Ferenc1, Klaus Pfizenmaier2, Angelika Hausser2, Katalin Schlett1,2

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Dendritic Filopodia Motility Analyser is a user-friendly, efficient InageJ/Fiji plugin, which allows the semi-automated quantitative evaluation of dendritic filopodia or spine motility. Our method is based on the pixel intensity dependent calculation of weighted center of mass of dendritic protrusions.

If you are using the plugin, please cite the following paper, in which the program and the analysis are described:

Tárnok K, Gulyás M, Bencsik N, Ferenc K, Pfizenmaier K, Hausser A, Schlett K. A new tool for the quantitative analysis of dendritic filopodial motility. (2015) Cytometry A. 87(1):89-96. doi: 10.1002/cyto.a.22569.

Prospective users should read this paper before running the program.

Terms of Use

This program is free software; you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation; either version 3 of the License, or (at your option) any later version. This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details. You should have received a copy of the GNU General Public License along with this program. If not, see Copyright © 2014 Márton Gulyás


DFMA_.jar (version 1.73, 25 April 2014)

Download sample image dataset

Registered sample.

Original sample.


ImageJ 1.48 or Fiji with ImageJ 1.48 or higher. (Other versions should also work, but have not been tested)


Download the DFMA_.jar file and copy it to the ‘\Plugins’ folder of Fiji or ImageJ. Restart Fiji/ImageJ and use the “Dendritic Filopodia Motility Analyser” item within the Plugins menu.